Rescue of recipient MSC functions and remission of recipient osteoporosis by donor MSC-derived exosomes through transfer of miR-26a
10.3969/j.issn.1001-3733.2017.05.001
- VernacularTitle:供体MSCs来源的外泌体转运miR-26a恢复宿主MSCs功能并缓解骨质疏松
- Author:
Qingyuan YE
1
;
第四军医大学学员一旅
;
Xinyu QIU
;
Rong TIAN
;
Shiyu LIU
Author Information
1. 710032西安,军事口腔医学国家重点实验室,口腔疾病国家临床医学研究中心,陕西省口腔疾病国际联合研究中心,第四军医大学口腔医院组织病理学教研室;第四军医大学学员一旅
- Keywords:
Mesenchymal stem cells (MSCs);
Osteoporosis;
Exosomes
- From:
Journal of Practical Stomatology
2017;33(5):575-579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore mechanisms underlying the rescuing effects of transplanted mesenchymal stem cells (MSCs) derived exosomes on estrogen-deficient osteoporosis.Methods:Mouse estrogen-deficient osteoporosis model was constructed in 12 female C57BL6/J rats and the exosome release was regulated by siRNA.Osteogenic induction,alizarin red staining and qPCR were performed to evaluate the effects of exosomes on recipient MSC functions.The miR-26a mimics and inhibitors and qPCR were used to explore the mechanisms underlying exosome-mediated functional rescue of recipient MSCs.Results:Donor MSCs alleviated estrogen-deficient osteoporosis via exosome release,and the alleviated osteoporosis by exosomes rescued the recipient MSC functions were observed.Moreover,the rescued recipient MSC functions by exosomes transferred miR-26a were found.Conclusion:Donor MSC-derived exosomes may rescue MSC functions and may remit osteoporosis of the recipient through transfering miR-26a.
