Effect of reactive nitrogen metabolite scavengers on sensitivity of human leukemia cells to immunotherapy
10.3760/cma.j.issn.1008-1372.2017.10.011
- VernacularTitle:反应性氮代谢物清除剂对人白血病细胞免疫治疗敏感性影响的实验研究
- Author:
Huaping XIAO
1
;
65212 美国密苏里州哥伦比亚,美国密苏里大学医学院Ellis FISCHEL肿瘤中心
;
Hui XIE
;
Chunyang LUO
;
Qing LI
;
Yujiang FANG
Author Information
1. 423000湖南省郴州,湘南学院附属医院肿瘤防治中心;65212 美国密苏里州哥伦比亚,美国密苏里大学医学院Ellis Fischel肿瘤中心
- Keywords:
Thiopronine/PD;
Leukemia/TH;
Immunotherapy
- From:
Journal of Chinese Physician
2017;19(10):1496-1499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Tiopronin (TIP) on interleukin (IL)-2 immunotherapy of human leukemia KG-1 cells and its possible mechanism.Methods KG-1 ceils in logarithmic growth phase were randomly divided into KG-1 + IL-2 group and KG-1 + IL-2 + TIP group.Methyl thiazolyl tetrazolium (MTI) assay and colony formation assay were used to detect the sensitivity and proliferation of KG-1 cells.The changes of reactive nitric metabolites (RNM) were detected with nitrate reductase method.The production of tumor necrosis factor (TNF)-3 and interferon (IFN)-γ,was detected with enzyme linked immunosorbent assay (ELISA).The expression of CD3ξ was detected with Western blot and real time polymerase chain reaction (RT-PCR).Results IL-2 and IL-2 + TIP could inhibit the growth of KG-1 cells.The inhibitory rate of KG-1 + IL-2 + TIP group was significantly higher than that of KG-1 + IL-2 group,and the sensitivity of KG-1 cells to IL-2 was 6.2 times higher.Both IL-2 and IL-2 + TIP group inhibited the colony formation of KG-1 cells.Compared to KG-1 + IL-2 group,KG-1 + IL-2 + TIP group inhibited the colony formation of KG-1 cells by 3.5 times.The RNM production of KG-1 + IL-2 group was (158.26 ± 3.82) μmol/ml,which was significantly higher than (45.18 ± 4.29) μ mol/ml of KG-1 + IL-2 + TIP group (P ＜ 0.05).The levels of TNF-β and IFN-γin KG-1 + IL-2 + TIP group were (253.28 ± 7.84) pg/ml and (181.25 ±6.41) pg/ml,which was significantly higher than (98.45 ±6.43) pg/ml and (68.74 ±8.26) pg/ml of KG-1 +IL-2 group (P＜0.05).The expression of CD3ξ in KG-1 +IL-2 +TIP group was significantly higher than that in KG-1 + IL-2 group.Conclusions Tiopronin can promote NK/T cell activity and increase the sensitivity of leukemia KG-1 cells to IL-2 by eliminating reactive nitrogen metabolites.
