Anti-acetylcholinesterase activity of the aglycones of phenolic glycosides isolated from Leonurus japonicus
- Author:
Nugroho AGUNG
1
;
Choi Sue JAE
;
Hong JOON-PYO
;
Park HEE-JUHN
Author Information
1. Department of Agro-industrial Technology
- Keywords:
Leonurus japonicus;
Lamiaceae;
Acetylcholinesterase;
Phenolic glycosides;
Quercetin;
Caffeic acid
- From:Asian Pacific Journal of Tropical Biomedicine
2017;7(10):849-854
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To find the genuine structure with anti-acetylcholinesterase(anti-AChE)from the phenolic glycosides abundant in Leonurus japonicus(Lamiaceae).The assay for anti-AChE activity is often used to lead anti-Alzheimer's drugs. Methods: The five phenolic glycosides, tiliroside, leonurusoside C, 2'''-syringoylrutin, rutin, and lavanduliofolioside were isolated from L. japonicus. The activities of the glycosides were relatively low. Seven compounds including p-coumaric acid, caffeic acid, hydroxytyrosol, salidroside, syringic acid, kaempferol, and quercetin, which are produced by the hydrolysis of the five glycosides, were also assayed for anti-AChE activity. Results: Of those seven compounds, the five compounds other than salidroside and syringic acid exhibited potent anti-AChE activities.In particular,the IC50s of caffeic acid and quercetin were (1.05 ± 0.19)and (3.58 ± 0.02)μg/mL, respectively. Rutin was the most abundant flavonoid in the extract(9.18 mg/g as measured by HPLC). Conclusion: The substances with potent anti-AChE were caffeic acid, quercetin, p-coumaric acid, kaempferol, and hydroxytyrosol that can be produced from their glycosides.
