Expression of LP-PLA2 mRNA in peripheral blood monocytes subsets of patients with type 2 diabetes mellitus
10.3760/cma.j.issn.1000-6699.2017.10.003
- VernacularTitle:2型糖尿病患者外周血单核细胞亚群脂蛋白相关磷脂酶A2基因表达研究
- Author:
Mingzhong SUN
1
;
Zhongwei ZHOU
;
Yuqiao JI
;
Hui ZHU
;
Dongmei JIANG
;
Hongmei CHEN
Author Information
1. 224001,东南大学医学院附属盐城医院检验科
- Keywords:
Diabetes mellitus;
type 2;
Monocyte subsets;
Lipoprotein phospholipase A2;
Atherosclerosis
- From:
Chinese Journal of Endocrinology and Metabolism
2017;33(10):822-827
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of monocyte subsets in the pathogenesis of type 2 diabetes mellitus(T2DM), the expression was detected on lipoprotein-associated phospholipase A2(LP-PLA2) mRNA in peripheral blood monocytes subsets of type 2 diabetic patients. Methods After the peripheral blood of 32 type 2 diabetic patients and 22 healthy controls were collected,the percentages of the monocytes subsets were detected by flow cytometry. The expression of LP-PLA2 mRNA in monocytes subsets were detected by PCR. After monocyte subsets of healthy subjects were induced into macrophages and stimulated by the serum of type 2 diabetic patients for 24h,LP-PLA2 mRNA expression was detected. Results Compared with the control group, the percentage of classical monocytes in T2DM group was decreased, while the LP-PLA2 mRNA expression and percentage of nonclassical,intermediate monocytes were significantly increased(all P<0.05). In addition,the homeostasis model assessment for insulin resistance index was positively correlated with LP-PLA2 mRNA expression in non-classical(P<0.05)and intermediate monocytes(P<0.01)of type 2 diabetic patients. Compared with type 2 diabetic patients without carotid atherosclerosis, the levels of LP-PLA2 mRNA in intermediate and nonclassical monocytes were significantly higher than those in type 2 diabetic patients with carotid atherosclerosis(P<0.05). The level of LP-PLA2 mRNA in non-classical monocytes-derived macrophages stimulated with the serum of type 2 diabetic patients was significantly higher than that in the control group(P<0.05). Conclusion Monocytes subsets from type 2 diabetic patients are abnormally skewed toward to intermediate and non-classical monocytes with high expression of LP-PLA2 mRNA,which may play an important role in the pathogenesis of type 2 diabetes mellitus and atherosclerosis.
