Neuroprotective effect of LSS on Aβ1-42-induced BV-2 microglial cells through the inhibition of neuroinflammation
10.3867/j.issn.1000-3002.2017.10.097
- Author:
ZHAO YA
1
;
ZENG YUAN
;
QIN DA-LIAN
Author Information
1. Laboratory of Chinese Materia Medica
- Keywords:
LSS;
Aβ1-42;
BV-2;
neuroinflammation;
Alzheimer disease
- From:
Chinese Journal of Pharmacology and Toxicology
2017;31(10):1013-1013
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effect of neuroprotective effect of lychee seed saponins (LSS) in BV-2. METHODS Aβ1-42 induced BV-2 cells were incubated with LSS for 12 h, the content of the inflammatory factors such as IL-1β, TNF-α, COX-2 and iNOS in the supernatant of BV-2 cell were measured by ELISA. The detection of the mRNA levels and the protein expression of the inflammatory factors including IL-1β, TNF-α, COX-2 and iNOS using real-time PCR and Western blotting, respectively. RESULTS The level of IL-1β, COX-2 and iNOS significantly increased with the treatment of Aβ1-42, and 0.117 mg·L-1-0.469 mg·L-1 LSS can inhibit these increased level. CONCLUSION LSS conferred neuroprotection via inhibiting the inflammatory factors expression.
