Effect of dexmedetomidine on humoral immune function in septic mice
10.3760∕cma.j.issn.0254-1416.2017.10.030
- VernacularTitle:右美托咪定对脓毒症小鼠体液免疫功能的影响
- Author:
Qian TONG
1
;
Yulan LI
;
Xiaofang MA
;
Jingjing WANG
;
Yaojia HU
Author Information
1. 730000,兰州大学第一医院麻醉科
- Keywords:
Dexmedetomidine;
Sepsis;
Immunoglobin
- From:
Chinese Journal of Anesthesiology
2017;37(10):1271-1273
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of dexmedetomidine on humoral immune function in septic mice. Methods Ninety SPF healthy male BALB∕c mice, aged 1 month, weighing 18-22 g, were divided into 3 groups(n=30 each)using a random number table: sham operation group(group S), sep-sis group(group SEP)and dexmedetomidine group(group DEX). Sepsis was induced by cecal ligation and puncture in SEP and DEX groups, and group S only underwent exploratory laparotomy. Dexmedetomi-dine 30 μg∕kg was intraperitoneally injected immediately after peritoneum closure in group DEX, and the e-qual volume of normal saline was given instead in S and SEP groups. The orbital venous blood samples were collected at 6, 12 and 24 h after operation(T1-3)for determination of serum concentrations of IgG, IgA, IgM and complement C3 and C4 by enzyme-linked immunosorbent assay. Then the spleen was removed, and the proliferation of spleen B lymphocytes was detected by methyl thiazolyl tetrazolium assay. Results Compared with group S, the serum IgG and IgM concentrations and proliferation of spleen B lymphocytes were significantly decreased at T2,3, the proliferation of spleen B lymphocytes was enhanced at T1, the ser-um concentrations of complement C3 and C4 were increased at T1-3(P<005), and no significant change was found in serum IgA concentrations in SEP and DEX groups(P>005). Compared with group SEP, the serum concentrations of IgG and IgM were significantly increased at T2,3, the proliferation of spleen B lymphocytes was enhanced at T1,2, the serum concentrations of complement C3 and C4 were decreased at T1-3(P<005), and no significant change was observed in serum IgA concentration in group DEX(P>005). Conclusion Dexmedetomidine can improve humoral immune function in septic mice.
