Preparation and application of magnetic molecularly imprinted nanoparticles for separation and purification of protein
10.7652/jdyxb201801027
- VernacularTitle:分子印迹磁性纳米粒的制备及其在蛋白质分离纯化中的应用
- Author:
shuang Xiao TANG
1
;
Li WANG
;
cen Si WANG
Author Information
1. 西安交通大学第二附属医院
- Keywords:
protein;
separation;
purification;
molecularly imprinted polymer
- From:
Journal of Xi'an Jiaotong University(Medical Sciences)
2018;39(1):127-130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a novel strategy for separation and purification of target protein by magnetic molecularly imprinted nanoparticles . Methods Fe3 O4 nanoparticles were synthesized through a hydrothermal method .A sol-gel technique was adopted to prepare imprinting layers .The adsorption capability of magnetic molecularly imprinted nanoparticles was investigated by kinetic , isothermal , and selective binding experiments .Results The imprinted nanomaterials could reach equilibrium within 30 min . The adsorption capacity was 44 .51 mg/g .The imprinting factor and selectivity coefficient were 3 .50 and 2 .92 ,respectively .The resulting imprinted polymers could selectively separate and enrich bovine hemoglobin from a bovine blood sample . Conclusion The prepared imprinted nanomaterials with good specific adsoprtion ability can provide an effective strategy for separation and purification of target protein .
