Effect of BMSCs transplantation on expressions of miR-34a and miR-21 in rat cerebral ischemia/reperfu-sion injury
10.3969/j.issn.1006-5725.2017.23.006
- VernacularTitle:骨髓间充质干细胞移植对缺血再灌注脑组织miR-34a、miR-21表达的影响
- Author:
Chunlan HUANG
1
;
Yan TANG
;
Yonghong TANG
Author Information
1. 南华大学附属第二医院神经内科
- Keywords:
cerebral ischemia/reperfusion;
BMSCs transplantation;
miR-34a;
miR-21;
neuro-pro-tective effect
- From:
The Journal of Practical Medicine
2017;33(23):3871-3875
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuro-protective effect of bone marrow mesenchymal stem cells (BMSCs)transplantation in cerebral ischemia/reperfusion injury model. Methods The modified Zea-Longa suture method was adopted to establish rat middle cerebral artery occlusion(MCAO)/reperfusion injury model.Experimen-tal rats were randomly divided into blank group,model group,PBS group and BMSCs transplantation group. PBS group and BMSCs transplantation group were respectively received tail vein injection of 1 mL PBS or BMSCs(l × l06)6 h after the establishing of MCAO/reperfusion model. Neurological function was evaluated using the modified neurologic severity score(mNSS)scale at 12 h,1 d,3 d and 7 d after operation.Quantitative real-time PCR was used to detect the levels of miR-34a and miR-21 in ischemic brain tissue. Results(1)The mNSS scores in BM-SCs transplantation group showed no significant difference when compared with those in model group at 12 h and 1 d after operation(P > 0.05),whereas were significantly lower than those in model group at 3 d and 7 d(P <0.05).(2)Compared with model group,BMSCs transplantation group showed decreased level of miR-34a(P <0.01),while elevated level of miR-21 at different time points(P<0.01). Conclusions BMSCs transplantation can improve the impaired neurological function induced by cerebral ischemia/reperfusion injury,showing neuro-pro-tective effect.The mechanism may be associated with regulating the expressions of apoptosis-related microRNAs.
