The relationship between differentially expressed miR-19b-3p and cognitive function in patients with Alzheimer's disease
10.3760/cma.j.issn.1674-6554.2017.11.007
- VernacularTitle:阿尔茨海默病患者血清miR-19b-3p表达与认知功能的关系
- Author:
Jing XU
1
;
Yuquan WU
;
Juan XU
;
Yi DAI
;
Demin JIAO
;
Jinhua HU
;
Xiaona CHEN
Author Information
1. 中国人民解放军第一一七医院老年病诊治中心
- Keywords:
Alzheimer's disease;
Serum;
miR-19b-3p;
β-amyloid precursor protein cleavage enzyme 1
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(11):994-998
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the relationship between differentially expressed miR-19b-3p and cognitive function in patients with Alzheimer's disease (AD).Methods The miRNA expression profiles of patients with AD(AD group,n=30) or healthy elderly people (NC group,n=30) were analyzed by Illumina/Solexa high-throughput sequencing technique.The miRNA lentiviral plasmids were constructed and injected into the model of AD rats.The cognitive function of rats was analyzed with water maze test.The mimics and inhibitors were synthesized and transfected into SH-SY5Y cell lines.The protein expression of β-amyloid precursor protein cleavage enzyme 1 (BACE1) was detected by western blot.Results Compared with the NC group,the expression of miR-146a-5p (log2AD/Control =2.3),miR-195-5p (log2 AD/Control =10.2),miR-20b-5p(log2AD/Control =15.1) in serum of AD group was up-regulated and the expression of miR-19b-3p (log2 AD/Control =-8.0) and miR-125b-3p (log2 AD/Control =-15.3) was down-regulated (P＜ 0.05).Compared with the rats in the AD group((26.50±3.12) s),the rats in the AD+miR-19b-3p group ((15.33±2.78) s) showed significantly shorter escape latencies(P＜0.05).Compared with the NC group,the protein expression of BACE1 in miR-19b-3p mimetic group was decreased and the protein expression of BACE1 in inhibitor group was increased.Conclusion miR-19b-3p may improve the cognitive function of AD patients via regulating the expression of BACE1.
