Interaction of oleanolic acid and its derivatives with bovine serum albumin by spectrofluorimetry
10.11665/j.issn.1000-5048.20170511
- VernacularTitle:齐墩果酸及衍生物与牛血清白蛋白相互作用的荧光光谱分析
- Author:
Tianxing ZHANG
1
;
Wei HUANG
;
Xueyu HUANG
;
Anping LIAO
Author Information
1. 广西民族大学广西多糖材料与改性重点实验室培育基地/广西高校化学与生物转化过程新技术实验室
- Keywords:
oleanolic acid;
derivatives;
bovine serum albumin;
interaction;
spectrofluorimetry
- From:
Journal of China Pharmaceutical University
2017;48(5):572-576
- CountryChina
- Language:Chinese
-
Abstract:
Oleanolic acid was chemically modified to improve its interaction with bovine serum albumin,thus to increase the mutual binding ability.As a lead compound,oleanolic acid reacted with Jones-reagent,then methyl iodide and finally hydroxylamine to obtain four different derivatives.Their structures were confirmed by IR and 1H NMR analysis.The interaction between these derivatives and bovine serum albumin and the effect of temperature and trace metal ions as Cu2+ and Co2+ on the interaction were investigated by fluorescence spectroscopy.All these derivatives exhibited improvement of the interaction compared with oleanolic acid with 16 times stronger for OA2.The interaction also increased with the presence of trace metal ions as Cu2+ and Co2+.These results indicate that chemical modification can improve the interaction of oleanolic acid with bovine serum albumin.
