Study on the value of PCR for rapid screening Salmonella/Shigella in preventive health physical examination
10.3969/j.issn.1673-4130.2017.23.018
- VernacularTitle:预防性健康体检快速筛查沙门志贺氏菌的价值研究
- Author:
Sufen ZHU
1
;
Hanqing TAN
Author Information
1. 广宁县疾病预防控制中心检验科
- Keywords:
Salmonella;
Shigella;
PCR;
health physical examination;
clinical value
- From:
International Journal of Laboratory Medicine
2017;38(23):3268-3269,3273
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the clinical value of PCR method for rapid screening Salmonella/Shigella in health physical examination .Methods A total of 3256 cases who were taken health physical examination from July 2011 to June 2015 ,were select-ed as the research object .Real-time fluorescence quantitative PCR method and the culture method were used for detecting Salmonel-la/Shigella ,and two methods were compared on their strengths and weaknesses for screening Salmonella/Shigella .Results A total of 46 cases with Salmonella were detected by PCR ,and the ratio was 1 .41% ;41 cases with Salmonella were detected by traditional culture method ,and the ratio was 1 .26% .39 cases with Shigella were detected by PCR and ,the ratio was 1 .20% ;32 cases were de-tected by traditional culture method ,and the ratio was 0 .98% .The difference was statistically significant (P<0 .05) .Sensitivity and specificity of PCR to detect Salmonella were 100 .00% and 99 .84% ,respectively ,and sensitivity and specificity of PCR to detect Shigella were 100 .00% and 99 .78% ,respectively .The PCR method is better than the traditional culture method concerning man-power ,processing time ,and satisfaction of test-takers ,and the cost of PCR method is significantly higher than that of traditional culture ,with a significant difference (P<0 .05) .PCR method contains high technology and has strong objectivity .Conclusion Real time fluorescent quantitative PCR method ,with high efficiency ,can be employed well for screening intestinal tract pathogenic bacte-ria ,improve the detection rate and accuracy for detecting Shigella and Salmonella ,and save time and energy as well .
