CD4+T cells mediate respiratory syncytial virus(RSV)-induced airway inflammation through secre-ting Th2 cytokines
10.3760/cma.j.issn.0254-5101.2017.10.005
- VernacularTitle:CD4+T细胞通过分泌Th2型细胞因子介导RSV感染所诱发的气道炎症反应
- Author:
Song BAI
1
;
Yulin CUI
;
Ruonan CHAI
;
Yue FAN
;
Beixing LIU
Author Information
1. 中国医科大学基础医学院免疫学教研室
- Keywords:
Respiratory syncytial virus;
CD4+T cell;
Airway inflammation
- From:
Chinese Journal of Microbiology and Immunology
2017;37(10):747-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of CD4+T cells in airway inflammation induced by respiratory syncytial virus (RSV) infection. Methods Animal models of acute RSV infection were estab-lished. Lung tissues were stained with hematoxylin and eosin (HE) to observe histopathological changes. Total number of CD4+T cells and the number of CD4+T cells secreting Th1/Th2 cytokines (IFN-γ, IL-4, IL-5 and IL-13) in spleen were detected by flow cytometry. Adoptive transfer of CD4+T cells was performed to identify the role of CD4+T cells in RSV-induced airway inflammation. Results RSV infection increased the total number of splenic CD4+T cells,particularly Th2-type CD4+T cells. The absolute numbers of IL-4/IL-5/IL-13-secreting CD4+T cells were increased significantly after RSV infection. Furthermore, adoptive transfer of CD4+T cells into BALB/c mice not only promoted the infiltration of mononuclear cells in lung,but also enhanced the secretion of Th2 cytokines during RSV infection. Conclusion CD4+T cells are involved in RSV-induced airway inflammation through secreting Th2 cytokines.
