Effect of brain-derived neurotrophic factor on the NFAT3/c4 signaling pathway after whole brain radiotherapy
10.3760/cma.j.issn.1004-4221.2017.12.018
- VernacularTitle:BDNF对全脑放疗后NFAT3/c4介导的信号通路相关因子作用研究
- Author:
Meiling XU
1
;
Junjun ZHANG
;
Qixian ZHANG
;
Haohao WU
;
Qiuhong FAN
;
Ye TIAN
Author Information
1. 215004,苏州大学附属第二医院放射治疗科 苏州大学放射肿瘤治疗学研究所 苏州市肿瘤放射治疗学重点实验室
- Keywords:
NFAT3/c4;
Irradiation;
Hippocampal;
Rat
- From:
Chinese Journal of Radiation Oncology
2017;26(12):1438-1442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of various radiation doses on the NFAT3/c4 signaling pathway and the improvement effect of exogenous brain-derived neurotrophic factor(BDNF)on this pathway. Methods Four groups of one-month-old Sprague-Dawley rats received radiation doses of 0,2,10,and 20 Gy, respectively, in a single radiation. At three days after radiation, exogenous BDNF was injected stereotaxically into the bilateral hippocampus. Western blotting and RT-PCR were used to assess the levels of NFAT3/c4-related proteins in the hippocampus. Results The results of Western blotting and RT-PCR showed that the level of NFAT3/c4 was reduced in a dose-and time-dependent manner after ionizing radiation. Compared with the radiation alone group,the ionizing radiation plus BDNF group had significantly increased levels of NFAT3/c4 and CaN with increases in radiation dose and time. Conclusions Whole brain radiotherapy inhibits the CaN/NFAT3/c4 signaling pathway. Exogenous BDNF can promote the NFAT-dependent transcription and then improve the cognitive function.
