Effects of Astragali Radix Injection Combined with Entecavir on Serum Inflammatory Cytokines and Liv-er Function of Model Mice with Hepatitis B
10.6039/j.issn.1001-0408.2017.34.12
- VernacularTitle:黄芪注射液联合恩替卡韦对乙型肝炎模型小鼠血清炎症因子及肝功能的影响
- Author:
Xueyuan HU
1
;
Xingying LI
;
Qin YANG
;
Lizhong HUANG
Author Information
1. 重庆三峡医药高等专科学校中医系
- Keywords:
Astragali radix injection;
Entecavir;
Hepatitis B;
Inflammatory cytokines;
Liver function;
Mice
- From:
China Pharmacy
2017;28(34):4800-4803
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the effects of Astragali radix injection combined with entecavir on serum inflammatory cyto-kines and liver function of model mice with hepatitis B. METHODS:60 mice were randomly divided into normal control group (normal saline),model group (normal saline),Astragali radix injection group (0.5 mL/10 g),entecavir group (45 μg/kg) and combination group(0.5 mL/10 g Astragali radix injection+45 μg/kg entecavir),12 in each group. Except for normal control group, mice in other 4 groups were induced for hepatitis B models. After modeling,all mice were intragastrically administrated once a day,for 4 weeks. After administration,tumor necrosis factor α(TNF-α),interferon γ(IFN-γ),interleukin 8 (IL-8),aspartate aminotransferase(AST),alanine aminotransferase(ALT),total bilirubin(TBIL)levels in serum were detected,and the pathologi-cal changes in liver tissue in each group were observed. RESULTS:Compared with normal control group,TNF-α,IFN-γ,IL-8, AST,ALT,TBIL levels in serum of mice in model group were significantly increased(P<0.05);and liver cells were diffuse and severe steatosis. Compared with model group,TNF-α,IFN-γ,IL-8,AST,ALT,TBIL levels in serum of mice in each administra-tion group were significantly decreased(P<0.05),pathological changes in liver tissue were improved to varying degrees,and the index improvement degree in combination group was superior to Astragali radix injection group and entecavir group (P<0.05). CONCLUSIONS:Astragali radix injection combined with entecavir helps to down-regulate the expressions of inflammatory cyto-kines of model mice with hepatitis B,and improve the liver function. The combination use has better effect than single use.
