Bioinformatics analysis of radioresistance key gene and pathway in esophageal carcinoma
10.3760/cma.j.issn.0254-5098.2017.10.002
- VernacularTitle:食管癌放射抗拒关键基因及通路的生物信息学分析
- Author:
Ya GUO
1
;
Hongbing MA
;
Baofeng WANG
;
Hongtao REN
Author Information
1. 710004,西安交通大学第二附属医院肿瘤科
- Keywords:
Esophageal carcinoma;
Radioresistance;
Gene expression omnibus;
Bioinformatics
- From:
Chinese Journal of Radiological Medicine and Protection
2017;37(10):730-735
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore key gene and pathway of radioresistance in esophageal carcinoma and reveal its molecular mechanism of radioresistance. Methods The gene expression profiles of GSE61772 and GSE61620 were downloaded from the Gene Expression Omnibus (GEO). Differentially expressed genes ( DEGs ) were screened by GEO2R. Gene Ontology ( GO ) enrichment, Kyoto Encyclopedia of Genes and Genomes ( Kegg ) enrichment and protein-protein interaction ( PPI ) network construction were performed by DAVID and String softwares. RT-PCR was used to detect the differences in the expression of different genes in different radiosensitivity cells. Results A total of 49 differentially expression genes were screened. These genes were mainly involved in the regulation of multicellular biosynthesis, ion transport, DNA synthesis, metabolism, cell proliferation and so on. The major biological pathways included a Wnt signal pathway. 12 DEGs interacted with each other, and CHN2 may be a key node. The expression of CHN2 gene had no obvious difference between TE13R and TE13. Conclusions 49 differentially expressed genes, including CHN2, may be involved in radioresistance of esophageal carcinoma, and the Wnt signaling pathway may be an important pathway in this radioresistance.
