Effects of Jatrorrhizine on Akt/AMPK/eNOS Signaling Pathways in Blood Vessel of Diabetes Rats
10.3870/j.issn.1004-0781.2017.10.005
- VernacularTitle:药根碱对糖尿病模型大鼠血管Akt/AMPK/eNOS信号通路的影响
- Author:
Yunshan WANG
1
;
Hong ZHANG
;
Xiaochun ZHANG
Author Information
1. 湖北医药学院附属随州医院药剂科
- Keywords:
Jatrorrhizine;
Diabetes;
Anti-inflammation;
Nitric oxide synthase;
endothelidal
- From:
Herald of Medicine
2017;36(10):1107-1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of jatrorrhizine on the Akt/AMPK/eNOS signaling pathways and potential protective function in blood vessel of diabetes rats. Methods Male Wistar rats ( n=60) were randomly divided into normal control group, model control group, low-and high dose jatrorrhizine groups. Except normal control group, the other rats were given intraperitoneal injection of STZ after induced insulin resistance, to made typeⅡdiabetes model. CMC-Na solution (5%) was given to normal control and model control group, and the jatrorrhizine resolved in the same solution was administered to low (50 mgkg-1) and high dose (100 mgkg-1) jatrorrhizine groups for 8 weeks. Their body weight, blood glucose, and seruminsulin levels were measured at the end of the treatment, IL-1β, TNF-αlevel in serum were measured by ELISA, and the eNOS, Akt/AMPK protein expression levels in the blood vessel were measured by Western blotting. Results Compared with normal control group, the weight of model control gropwas lossed, blood glucose was increased(P<0.01). Compared with model control group, high-dose jatrorrhizine significantly increased body weight, alleviated blood glucose and decreased serum insulin ( P<0.01) . Serum inflammatory factor like IL-1βwas (92.3±4.3) pgmL-1 in normal control group, (152.4±20.0) pgmL-1 in model control group, (120.96±33.0) pgmL-1 and (95.05±7.7) pgmL-1 in low-and high-dose jatrorrhizine groups, respectively. TNF-αwas (10.50±0.82) pgmL-1 in model control group, (7.48±0.36) pgmL-1 in normal control group, (8.82±0.42) and (7.11±0.33) pgmL-1 in low- and high- dose jatrorrhizine groups, respectively. As compared with control group, eNOS and Akt/AMPK expression in blood vessel was significantly reduced (P<0.05) in model control group, and those were significantly increased in high-dose jatrorrhizine group as compared with model control group ( P<0.05 or P<0.01) . Conclusion Jatrorrhizine may exert protective effect on diabetes mellitus rats through regulating Akt/AMPK/eNOS signaling pathway in blood vessel.
