Determination of 18β-isomer in Magnesium Isoglycyrrhizinate by HPLC
10.3870/j.issn.1004-0781.2017.10.022
- VernacularTitle:高效液相色谱法测定异甘草酸镁中18β-异构体的含量
- Author:
Shanchun WANG
1
;
Hongmei GU
;
Xiquan ZHANG
;
Zhilin CHEN
;
Yang LI
;
Jun LI
Author Information
1. 正大天晴药业集团股份有限公司研究院
- Keywords:
Magnesium isoglycyrrhizinate;
18β-isomer;
Chromatography,high performance liquid;
Content determination
- From:
Herald of Medicine
2017;36(10):1179-1182
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for determination of 18β-isomer in magnesium isoglycyrrhizinate. Methods The determination was performed by Agilent Extend-C18 column ( 4. 6 mm × 250 mm, 5 μm ) . Mobile phase consisted of 0. 1 mol·L-1 potassium dihydrogen phosphate buffer solution ( adjusted to pH 7. 0 with potassium hydroxide )-acetonitrile (80︰20) at the flow rate of 1.0 mL·min-1. The column temperature was 30 ℃, and the detection wavelength was set at 250 nm. Results The resolution of magnesium isoglycyrrhizinate and 18β-isomer was greater than 2.0. The linear range of them was 0.41-2.46μg·mL-1( r=0.9998) , the detection limit was 0.21 ng, and the average recovery were 100.2%,99.1%, 110.2%,RSD were 0.9%,0.1%,0.2%(n=3). Conclusion The method is simple and accurate, and can be used for determination of 18β-isomer in magnesium isoglycyrrhizinate.
