Composition and clinical significance of γδ T lymphocytes in peripheral blood of Gestational Diabetes Mellitus patients
10.3969/j.issn.1673-4130.2017.18.001
- VernacularTitle:妊娠糖尿病患者外周血γδ T细胞的改变及临床意义
- Author:
Yumei DAI
1
;
Xueying LING
;
Yanmei LI
;
Yunfeng LIU
;
haiying LIU
Author Information
1. 广州医科大学附属广州市妇女儿童医疗中心临床检验部
- Keywords:
gestational diabetes mellitus;
γδ T cells;
flow cytometry
- From:
International Journal of Laboratory Medicine
2017;38(18):2497-2499
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the composition of γδ T cells in the peripheral blood of gestational diabetes mellitus(GDM) patients with those of normal pregnant women,so as to explore the association of γδ T cells with the onset and progression of GDM.Methods The peripheral blood mononuclear cells(PBMC) of GDM patients(case group) and normal pregnant women(con-trol group) were separated by Ficoll-Hypaque density gradient centrifugation.γδ T cells were labeled with FITC conjugated anti TCRγδ monoclonal antibody(McAb) and PE conjugated anti-CD3 McAb and analyzed by flow cytometry.Results γδ T cells in GDM patients' peripheral blood were about(6.89 ± 3.22) ％,which was higher than normal control group(4.26 ± 1.64) ％.The percentage of γδ T cells in non-treated GDM group was significantly increased to(8.79 ± 2.33)％,while in treated GDM group,the percentage of γδ T cells was drop to normal(3.76±1.62)％.Conclusion γδ T cells in GDM patients' peripheral blood and CD3+ T Cells were slightly increased.The alteration may be related to the onset of GDM.
