Active part of Glycyrrhiza extract induces apoptosis in human cervica l cancer HeLa cells and its related protein caspase-3 and caspase-9 expression
10.11904/j.issn.1002-3070.2017.05.002
- VernacularTitle:甘草提取物的有效部位诱导人宫颈癌HeLa细胞株凋亡及对凋亡相关蛋白Caspase-3和Caspase-9表达的影响
- Author:
Jixi WANG
1
;
Weisheng DENG
;
Fengru WANG
;
Zhimao SHI
;
Dan MENG
;
Qiang XU
;
Yuyao ZHANG
Author Information
1. 黑龙江中医药大学
- Keywords:
Licorice;
Anti-tumor effect;
Apoptosis;
HeLa cells;
Apoptosis protein
- From:
Practical Oncology Journal
2017;31(5):390-395
- CountryChina
- Language:Chinese
-
Abstract:
Objective The aim of this study was to determine whether active part of Glycyrrhiza extract (GL)induced apoptosis in Hela cells and its inhibitory mechanism.Methods HeLa cells were treated with 25 g/mL of GL for 24hs.Cell viability and apoptosis in HeLa cells were determined by MTT,AO/EB fluorescent double staining,transmission electron microscope(TEM),and Western blot.Results The MTT results showed that GL significantly inhibited the proliferation of HeLa cells in a dose-response.After treatment for 24 hrs,large number of early apoptotitc cell were observed using AO/EB fluorescent double staining and TEM.The expression of Pro-caspase-9 and Cleaved-caspase-3 protein was higher in GL-treated cells them those of the control cells(P<0.05).Conclusion GL can activate Caspase-3 and Caspase-9 genes to induce apoptosis in HeLa cells.
