Antitumor effect of allicin on esophageal cancer EC109 cells compared with chemotherapeutics in vitro
10.3969/j.issn.1000-4718.2017.10.018
- VernacularTitle:大蒜素与化疗药物对食管癌细胞EC109疗效比较的实验研究
- Author:
zhang Cheng CAO
1
;
qiao Shu HU
;
yin Hai LAI
;
jun Meng CHEN
;
zhen Yu SHI
;
Jian WU
Author Information
1. 福建医科大学附属龙岩第一医院心胸外科
- Keywords:
Allicin;
5-Fluorouracil;
Cisplatin;
Esophageal cancer;
Apoptosis
- From:
Chinese Journal of Pathophysiology
2017;33(10):1845-1851
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To investigate the inhibitory effect of allicin on human esophageal cancer cell line EC 109 and its possible mechanism by comparison with chemotherapeutic drugs .METHODS:The EC109 cells were treated with different concentrations of allicin , 5-fluorouracil and cisplatin .The cell viability was detected by CCK 8 assay and the activ-ity of lactic dehydrogenase (LDH) was analyzed at 6 h, 12 h, 24 h, 48 h and 72 h.The apoptosis of the EC109 cells in-duced by Z-VAD-FMK, allicin, allicin+Z-VAD-FMK, 5-fluorouracil and cisplatin was analyzed by flow cytometry with Annexin V-FITC and PI double staining .The enzyme activity changes of caspase-3, caspase-8 and caspase-9 were detected by spectrophotometry .RESULTS:Allicin had inhibitory effect on the growth of the EC 109 cells and killed them in con-centration-dependent and time-dependent manners .LDH activity was decreased compared with 5-fluorouracil and cisplatin . The increased activity of caspase-3 and caspase-8 in allicin-treated cells was statistically significant , but caspase-9 activity changed without statistical significance .CONCLUSION:Allicin inhibits the growth of EC109 cells in concentration-and time-dependent manners through extrinsic apoptosis pathways activated by caspase -8, and the side effects are weaker com-pared with 5-fluorouracil and cisplatin .
