Effects of Heyutai Fuzhu Jiangtang Tablets Combined with Metformin on Insulin Resistance in Skeletal Muscle of Diabetic Rats
10.3969/j.issn.1005-5304.2017.11.010
- VernacularTitle:和于泰辅助降糖片联合二甲双胍对糖尿病大鼠骨骼肌胰岛素抵抗的影响
- Author:
yuan Guang XU
1
;
Wen SUN
;
lin Zi SONG
;
Xuan GUO
;
li Li WU
;
ling Ling QIN
;
Dan HOU
;
Zhuo ZHANG
;
Shuo TIAN
;
Tong-hua XIANG
;
LIU LI
Author Information
1. 首都医科大学附属复兴医院
- Keywords:
Heyutai Fuzhu Jiangtang Tablets;
metformin;
combination-application;
PI3K/Akt signaling pathway;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2017;24(11):39-43
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of Heyutai Fuzhu Jiangtang Tablets combined with metformin in insulin resistance (IR); To discuss its mechanism of action. Methods 6–7 week old male ZDF (fa/fa) rats were randomly divided into model group,metformin group,Heyutai Fuzhu Jiangtang Tablets group(Jiangtang Tablets group),and metformin combined with Heyutai Fuzhu Jiangtang Tablets group.ZDF(fa/+)rats were chosen as normal group.Each medication group was given relevant medicine for gavage for 6 weeks. Body weight, FBG, TG, TC, FFA, FINS, HOMA-IR, OGTT and HE staining were tested. HE staining was used to observe the pathological changes of skeletal muscle. RT-PCR and Western blot were used to detect skeletal muscle corresponding gene and protein expression. Results Compared with Jiangtang Tablets group and metformin group, TC, FFA, FBG, and HOMA-IR in metformin combined with Heyutai Fuzhu Jiangtang Tablets group decreased significantly (P<0.05, P<0.01). Blood glucose level and AUC significantly decreased at each time point in OGTT. HE staining of skeletal muscle fibers arranged in order; nucleus increased and internal movement was not significant, without obvious infiltration of inflammatory cells. Expressions of skeletal muscle InsR, Akt, and Glut4 mRNA expression increased (P<0.05, P<0.01). Expressions of skeletal muscle p-InsR, p-Akt, and Glut4 protein expression increased (P<0.05, P<0.01). Conclusion Heyutai Fuzhu Jiangtang Tablets combined with metformin can improve IR in type 2 diabetic rats, and the effect is better than single-application.
