Circadian variations in salivary chromogranin a concentrations during a 24-hour period in dogs.
- Author:
Kazutaka KANAI
1
;
Mariko HINO
;
Yasutomo HORI
;
Ruriko NAKAO
;
Fumio HOSHI
;
Naoyuki ITOH
;
Seiichi HIGUCHI
Author Information
- Publication Type:Brief Communication
- Keywords: chromogranin A; circadian variations; dog; saliva
- MeSH: Animals; Chromogranin A/*analysis/*metabolism; *Circadian Rhythm; Dogs/*physiology; Saliva/*chemistry
- From:Journal of Veterinary Science 2008;9(4):421-423
- CountryRepublic of Korea
- Language:English
- Abstract: The purpose of this study was to determine if salivary chromogranin a secretion in dogs exhibits a circadian rhythm. Saliva sampling was performed during three different sessions occurring in three nonconsecutive 24-h periods. Sixteen healthy adult beagle dogs (8 males and 8 females) were moved to a sampling room and housed individually in cages. Saliva samples were obtained every 4 h from 12:00 p.m. to 12:00 p.m. the following day. In the interest of habituation, saliva was obtained hourly from each dog 3 h before the experiment was started. Salivary chromogranin A concentrations were measured using an enzyme-linked immunosorbent assay. No circadian rhythm was detected for salivary chromogranin A secretion, and no differences in salivary chromogranin A concentrations measured every 4 h were demonstrated during the 24-h cycle in dogs.
