The Effect of Hyaluronic Acid on the Expression of MMP-13 in Healing Process of Bone Defect in Rats.
10.4055/jkoa.2006.41.6.1021
- Author:
Ki Ser KANG
1
;
Han Jun LEE
;
Jin Woo LEE
;
Jae Sung LEE
;
Mi Kyung LEE
Author Information
1. Department of Orthopaedic Surgery, Chung-Ang University College of Medicine, Seoul, Korea. gustinoLHJ@hanafos.com
- Publication Type:Original Article
- Keywords:
Bone defect;
Healing;
MMP-13;
Hyaluronic acid
- MeSH:
Animals;
Bony Callus;
Chondrocytes;
Collagen Type II;
Femur;
Hyaluronic Acid*;
Osteoblasts;
Polymers;
Rats*;
Reverse Transcription
- From:The Journal of the Korean Orthopaedic Association
2006;41(6):1021-1027
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: Matrix metalloproteinase (MMP)-13 has a degradation effect on type II collagen and is expressed in osteoblastic cells and hypertrophic chondrocytes. However, the role of MMP-13 is not completely understood. This study investigated the effect of exogenous Hyaluronic acid (HA) on the expression of MMP-13 in the healing process of bone defects. MATERIALS AND METHODS: After making a bone defect in the metaphysis of the distal femur in rats, HA was instilled into the defect in the experimental group. In the control group, a bone defect was made but HA was not instilled. Two weeks after the experiment, the rats were sacrificed and the callus that filled the bone defects was obtained. A histological examination and real time reverse transcription polymeric chain reaction (RT-PCR) were performed to investigate the healing response and expression of MMP-13. RESULTS: Histologically, the proliferation of osteoblasts and the formation of osteoid were more active in the experimental group. Nevertheless, the level of MMP-13 expression was significantly lower in the experimental group than in the control group. CONCLUSION: From the results of this study, it appears that exogenous HA inhibits the expression of MMP-13 at the early stage of bone healing.
