Preliminary Examination for Apoptosis and Cell Proliferation Related to Aging Process in the Anterior Cruciate Ligament Cells of the Human Degenerative Arthritic Knee Joint.
10.4055/jkoa.2015.50.4.320
- Author:
Rak Min HYUN
1
;
Ki Won KIM
;
Seo Won JEONG
;
Jin Wha CHUNG
;
Hwa Sung LEE
Author Information
1. Department of Orthopaedic Surgery, Yeouido St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Seoul, Korea. hslee1003@catholic.ac.kr
- Publication Type:Original Article
- Keywords:
knee joint;
anterior cruciate ligament;
elderly;
apoptosis;
cell proliferation
- MeSH:
Aged;
Aging*;
Anterior Cruciate Ligament*;
Apoptosis*;
Arthroplasty;
Arthroplasty, Replacement, Knee;
Cell Proliferation*;
DNA Nucleotidylexotransferase;
Humans*;
Immunohistochemistry;
Knee Joint*;
Knee*;
Osteoarthritis
- From:The Journal of the Korean Orthopaedic Association
2015;50(4):320-327
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: We investigated the association between aging-induced apoptosis and cell proliferation in the anterior cruciate ligament (ACL) of aged patients. MATERIALS AND METHODS: Twenty patients with osteoarthritis who underwent total knee replacement arthroplasty were enrolled in the study. They were divided into three groups according to age: group A (< or =65 years), group B (66-75 years), and group C (> or =76 years). The ACL tissue was obtained intraoperatively and subjected to terminal deoxynucleotidyl transferase dUTP nick-end labeling staining and immunohistochemistry to quantify the apoptosis and cell proliferation indices. RESULTS: Apoptosis occurred in all groups, with the highest apoptosis index found in group C, followed by that in groups B and A. A statistically significant positive linear correlation was observed, with a 1-year increase in age resulting in an average increase of 1.49 in the apoptotic index. The lowest cell proliferation index was observed in group C, followed by that in group B and group A, with a 1-year increase in age resulting in an average decrease of 1.0 in the cell proliferation index, which was a statistically significant negative linear correlation. Consequently, a statistically significant negative correlation was confirmed between the apoptosis and cell proliferation indices, whereby an increase of 1.0 in the apoptosis index was concurrent with a decrease of 0.45 in the cell proliferation index. CONCLUSION: Apoptosis occurred in the ACL of human knee joint. With increasing age, apoptosis increased and cell proliferation decreased.
