Stimulation of bone formation in the expanding inter-premaxillary suture by vitamin E, in rat.
10.4041/kjod.2009.39.5.337
- Author:
Tancan UYSAL
1
;
Mihri AMASYALI
;
Huseyin OLMEZ
;
Yildirim KARSLIOGLU
;
Omer GUNHAN
Author Information
1. Department of Orthodontics, Faculty of Dentistry, Erciyes University, Kayseri, Turkey. tancanuysal@yahoo.com
- Publication Type:Original Article
- Keywords:
Vitamin E;
alpha-tocopherol;
Expansion;
Histomorphometry;
Rat
- MeSH:
alpha-Tocopherol;
Animals;
Osteogenesis;
Rats;
Rats, Wistar;
Retention (Psychology);
Sodium Chloride;
Sutures;
Vitamin E;
Vitamins
- From:Korean Journal of Orthodontics
2009;39(5):337-347
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: The aim of this study was to evaluate the effects of vitamin E (alpha-tocopherol) administration on bone formation in response to expansion of the inter-premaxillary suture, in rats, histomorphometrically. METHODS: Thirty 50 - 60 day old Wistar rats were separated into five equal groups (one control and four experimental). All groups were subjected to inter-premaxilla expansion with 50-gram of force. Six control animals received saline solution (Group I) and three experimental groups were treated with a single dose of alpha-tocopherol injected into the inter-premaxillary suture after one day after appliance placement (Group II: 2 mg/kg; Group III: 10 mg/kg; and Group IV: 50 mg/kg). A further group of six animals received three injections of 10 mg/kg alpha-tocopherol, one each on days 3, 6, and 9 (Group V). Bone formation in the suture was evaluated by bone histomorphometry. Kruskal-Wallis rank and Mann-Whitney U tests were used for statistical evaluation at p<0.05 level. RESULTS: New bone area, bone perimeter, feret's diameter and newly formed bone measurements were significantly higher in the experimental groups than the control (p<0.001). Bone architecture in alpha-tocopherol administrated groups was improved, and bone formation during the expansion period was stimulated significantly, in a dose-dependent manner. CONCLUSIONS: The application of alpha-tocopherol during the early stages to orthopedically expanded inter-premaxillary suture areas may stimulate bone formation and shorten the retention period, in rats.
