Effect of Dietary Conjugated Linoleic Acid (CLA) Isomers on Tumor Incidence and the Protein Expression of Cyclooxygenase-2 and Protein Kinase C in Colonic Mucosa of DMH-Treated Rats.
- Author:
Hyun Suh PARK
1
;
Chang Soo CHUN
;
Jung Han YOON
Author Information
1. Department of Food and Nutrition, Kyung Hee University, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
colon cancer;
CLA isomers;
cell proliferation;
cyclooxygenase-2;
protein kinase C
- MeSH:
1,2-Dimethylhydrazine;
Animals;
Anticarcinogenic Agents;
Body Weight;
Cell Proliferation;
Colon*;
Colonic Neoplasms;
Cyclooxygenase 2*;
Diet;
Dimenhydrinate;
Dinoprostone;
Humans;
Incidence*;
Injections, Intramuscular;
Linoleic Acid*;
Male;
Mucous Membrane*;
Prostaglandin-Endoperoxide Synthases;
Protein Kinase C*;
Protein Kinases*;
Rats*;
Rats, Sprague-Dawley
- From:The Korean Journal of Nutrition
2004;37(9):763-770
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
This study was designed to compare the anti-carcinogenic effect of conjugated linoleic acid isomers on tumor incidence, cell proliferation and the levels of thromboxane (TX)B2, prostaglandin (PG)E2 and 1,2-diacylglycerol (DAG), and the related enzyme expression of cyclooxygenase (COX)-2 and protein kinase C (PKC) in colonic mucosa of 1,2-dimethylhydrazine (DMH)-treated rats. One hundred eight male Sprague Dawley rats were randomly divided into 3 groups depending on the types of CLA isomers, i.e. control group (no CLA contained), c9t11 group (cis-9, trans-11 CLA contained), and t10c12 group (trans-10, cis-12 CLA contained). The experimental diet was composed of protein at 20%, carbohydrate at 56.2%, and fat at 14.5% including 1.0% CLA isomers by weight. The experimental diet was fed for 30 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total dose of 180 mg per kg body weight. Two CLA isomers (c9, t11; t10, c12) significantly reduced tumor incidence and cell proliferation by reducing the protein expression of COX-2 and PKC, and the level of TXB2, PGE2, and DAG in colonic mucosa. However, there was no significant difference in anti-carcinogenic effect between c9t11-CLA and t10c12-CLA.
