Effect of Polyinosinic-Polycytidylic Acid on MUC5B Expression in Human Airway Epithelial Cells.
10.3342/kjorl-hns.2015.58.9.615
- Author:
Yo Han CHOI
1
;
Chang Hoon BAE
;
Hyeong Geun KIM
;
Bo Hyeon SEO
;
Yoon Seok CHOI
;
Si Youn SONG
;
Yong Dae KIM
Author Information
1. Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
- Publication Type:Original Article
- Keywords:
Human airway epithelial cells;
Mitogen-activated protein kinase;
MUC5B;
Polyinosinic-polycytidylic acid
- MeSH:
Epithelial Cells*;
Humans*;
Immunoenzyme Techniques;
Mucins;
Mucus;
p38 Mitogen-Activated Protein Kinases;
Phosphorylation;
Poly I-C*;
Protein Kinases;
Real-Time Polymerase Chain Reaction;
RNA, Double-Stranded;
RNA, Messenger;
RNA, Small Interfering
- From:Korean Journal of Otolaryngology - Head and Neck Surgery
2015;58(9):615-621
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND AND OBJECTIVES: Polyinosinic-polycytidylic acid (Poly I:C) is structurally similar to double-stranded RNA, and is known to induce various inflammatory mediators and to cause inflammatory reactions in airway epithelial cells. However, the effect of Poly I:C on secretion of mucins in human airway epithelial cells has been very rarely reported. In this study, the effect and brief signaling pathway of Poly I:C on the expression of mucin genes were investigated in human airway epithelial cells. MATERIALS AND METHOD: In mucin-producing human NCI-H292 airway epithelial cells and the primary cultures of normal human nasal epithelial cells, the effect and signaling pathway of Poly I:C on expression of mucin genes were investigated using reverse transcriptase-polymerase chain reaction, real-time PCR, enzyme immunoassay, and immunoblot analysis with specific inhibitors and small interfering RNA (siRNA) for mitogen-activated protein kinase (MAPK). RESULTS: Poly I:C induced the MUC5B expression, and activated the phosphorylation of ERK1/2 and p38 MAPK. U0126 (ERK1/2 MAPK inhibitor) and SB203580 (p38 MAPK inhibitor) inhibited the Poly I:C-induced MUC5B expression. In addition, the knockdown of ERK2 and p38 MAPK by siRNA significantly blocked the Poly I:C-induced MUC5B mRNA expression. CONCLUSION: Poly I:C induces the MUC5B expression via ERK2 and p38 MAPK signaling pathways in human airway epithelial cells. Therefore, Poly I:C may play a role in the regulation of mucus hypersecretion through MAPK signaling pathways in the human airway epithelial cells.
