Evaluation of Platelet-Rich Plasma and Neutrophil Antimicrobial Extract as Two Autologous Blood-Derived Agents.
10.1007/s13770-017-0035-4
- Author:
Tomasz SZPONDER
1
;
Joanna WESSELY-SZPONDER
;
Anna SMOLIRA
Author Information
1. Department and Clinic of Animal Surgery, Faculty of Veterinary Medicine, University of Life Sciences, Głęboka 30, 20-612 Lublin, Poland. tomszpon@op.pl
- Publication Type:Original Article
- Keywords:
Antimicrobial peptides neutrophil extract;
Cathelicidin;
Platelet-rich plasma
- MeSH:
Cathelicidins;
In Vitro Techniques;
Intercellular Signaling Peptides and Proteins;
Leukocytes;
Macrophages;
Methods;
Neutrophils*;
Peptides;
Platelet-Rich Plasma*;
Respiratory Burst;
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization;
Superoxides
- From:
Tissue Engineering and Regenerative Medicine
2017;14(3):287-296
- CountryRepublic of Korea
- Language:English
-
Abstract:
The platelet-rich plasma (PRP) and antimicrobial peptides neutrophil extract (AMP extract) were prepared from rabbit neutrophils as two autologous blood-derived preparations, which could be applied locally to enhance healing process of tissues. Both preparations were analyzed using the MALDI TOF method for accurate qualitative assay. Growth factors (PDGF and VEGF) and microbicidal protein were reported in PRP. In AMP extract a-defensins, namely; NP-1, -2, -3a, -3b, -4, and -5 and cathelicidins represented among other by 15-kDa antibacterial protein (p15s) were detected. In the second part of our study the influence of antimicrobial extract on macrophages in vitro was tested. Then, degranulation of neutrophils in vitro and generation of reactive intermediates by these cells under the influence of AMP extract were assessed. As estimated, the addition of AMP extract into cultures of macrophages decreased superoxide anion generation after 5 days of incubation. Furthermore, AMP extract inhibited degranulation and respiratory burst in neutrophils, therefore in this regard it suppress proinflammatory effect of two studied populations of leukocytes.
