Fusion PCR for amplification of the full-length cDNA of dengue virus type 2 isolated in China
- VernacularTitle:扩增我国登革2型病毒全长cDNA分子的融合PCR方法
- Author:
Baochang FAN
;
Wei, ZHAO
;
ZhiJun, HU
;
Man, YU
;
Shuiping CHEN
;
PeiYing, YANG
;
EDe, QIN
- Publication Type:Journal Article
- From:Bulletin of The Academy of Military Medical Sciences
2001;25(2):137-139
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish fusion PCR for amplification of the full-length cDNA of dengue virus type 2. Methods:According to the published nucleotide sequence of D2-43,the primers were devised and the 5′ and 3′ half genomic cDNAs of dengue virus type 2 were amplified by long reverse transcription PCR. Using the PCR products as model,the approximate 11 kb full-length cDNA was amplified by fusion PCR. The sequence containing the 5′ noncoding region was determined by PRISMTM ABI 377 automated sequencer.Results:Using fusion PCR,the full-length cDNA of dengue virus type 2 was successfully amplified and its correctness was proved by partial nucleotide sequences analysis. To our best knowledge, this is the first report of the same kind.Conclusion:Fusion PCR is an effective method to amplify the genomic cDNA of dengue virus.
