Transient Expression of Hantaan Virus S segment Gene Coding region in vitro of Vero-E6 cells
- VernacularTitle:汉滩病毒S片段基因编码区在Vero-E6细胞中的表达
- Author:
Menghua, ZHANG
;
Hangyan, WANG
;
Weisong, YANG
;
Changxing, HUANG
;
Guangyu, LI
;
Yi, WANG
;
Xuefan, BAI
- Publication Type:Journal Article
- Keywords:
Hantaan virus;
Gene expression;
S segment;
Coding region
- From:Virologica Sinica
1999;14(2):181-
- CountryChina
- Language:Chinese
-
Abstract:
The coding region of S genome segment of Hantaan virus (76/118 strain) was inserted into the eukarytic expression plasmidpVR1012. The recombinant expression plasmid pVRS22 was constructed. Vero-E6 cells were transiently transfected in vitro with pVRS22 plasmid. The transient expression of Hantaan virus nucleocapsid proteins in Vero-E6 cells was detected by indirect immunofluorescence assay (IFA) with monoclonal antibody 5H5 against Hantaan virus.
