Effect of Stanozolol on Growth of Pubertal Rat Treated with Gonadotropin Releasing Hormone Agonist
- VernacularTitle:司坦唑醇对促性腺激素释放激素类似物注射后青春期大鼠生长的影响
- Author:
shun-ye, ZHU
;
zhen-hua, YU
;
hong-shan, CHEN
;
yan-hong, LI
;
si-nian, PAN
;
hua-mei, MA
;
min-lian, DU
- Publication Type:Journal Article
- Keywords:
stanozolol;
growth;
epiphyseal growth plate;
puberty;
gonadotropin releasing hormone;
rat
- From:Journal of Applied Clinical Pediatrics
2004;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of stanozolol(ST) on long bone growth and maturation of pubertal female rats treated with gonadotropin releasing hormone agonist(GnRHa).Methods At 3 weeks of age,42 female Sprague-Dawley rats(brood) were divided into 7 groups(ST dosage groups,as 5 000 ?g/100 g group,200 ?g/100 g group,100 ?g/100 g group,50 ?g/100 g group,25 ?g/100 g group,solvent control group and blank control group)(n=6).Forty-eight female rats were divided into 8 groups(ST therapeutic duration)(n=6).Rats received 2.5 mg/kg im slow-released GnRHa(triptorelin,as 2 d group,3 d group,5 d group,7 d group,10 d group,13 d group,soluent control group and blank control group) which was repeated every 2 weeks for 2 times,3 days after the 2nd GnRHa(D1),ST dosage groups were subcutaneously administrated ST at the various dosage daily(D1-D13).ST therapeutic duration groups were subcutaneously administrated ST at the dosage of 100 ?g/100 g daily for different duration.All the rats were killed on the D14.On the day of sacrifice,body weight,body length and left tibial length were measured,plasma were taken for determining insulin-like growth factor-1(IGF-1),right tibia were fixed,demineralized and processed for paraffin-embedding.Paraff sections were HE stained for growth plate measurements.proliferating cell nuclear antigen(PCNA) on growth plate was analyzed with immunohistochemistry staining and image.Results 1.In the 5 000 ?g/100 g ST dosage group,the weight,Height and tibial length exceeded than those of the other dosage and control groups(Pa
