The Gene Expression of Dynorphin, Enkephalin, c-Fos in the Neuropathic Rat.
10.4097/kjae.1996.31.3.293
- Author:
Neung Hee HAN
1
;
Won Hyung LEE
;
Won Jae HWANG
;
Sug Hwa YOON
;
Yong Sup SHIN
;
Soo Chang SON
;
Hye Ja KIM
;
Sae Jin CHOI
;
Kyu LIM
;
Byung Doo HWANG
Author Information
1. Department of Anesthesiology, School of Medicine, Chungnam National University, Taejeon, Korea.
- Publication Type:Original Article
- Keywords:
neuropathic pain;
c-fos;
dynorphin;
enkephalin
- MeSH:
Animals;
Blotting, Northern;
Dynorphins*;
Enkephalins*;
Gene Expression*;
Hyperalgesia;
Ligation;
Neuralgia;
Rats*;
RNA, Messenger;
Spinal Nerves;
Sympathectomy, Chemical
- From:Korean Journal of Anesthesiology
1996;31(3):293-303
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: We studied the time course of gene expression of dynorphin, enkephalin, c-fos, and the changes of allodynia, and the effect of chemical sympathectomy on the gene expression and allodynia in neuropathic rat. METHODS: In two groups of rat (Sprague-Dawley), the left L5 and L6 spinal nerves were tight ligated. In gene expression group (N=25), behavioral tests for mechanical allodynia and cold allodynia were perfomed for the next two weeks. After the test of allodynia, the expression of dynorphin, enkephalin, c-fos were assessed by Northern blot hybridization. In chemical sympathectomy group (N=16), after chemical sympathectomy (guanethidine 70 mg/kg intraperitoneally, from postoperative 7 days to 9 days), the changes of allodynia and the gene expression of enkephalin, c-fos were tested. RESULTS: Mechanical allodynia and cold allodynia was developed on the postoperative 3, 5, 7, 14 days. Preprodynorphin mRNA expression was reached peak level at the postoperative 8 hrs, sustained increase by the postoperative 3 days, but preproenkephalin mRNA expression increased slightly after operation. c-Fos mRNA expression was increased immediately at the postoperative 30 min, 1 hr, returned to normal level thereafter, and increased again on the postoperative 3, 5, 7 days that neuropathic pain was developed. Mechanical allodynia and cold allodynia were decreased by chemical sympathectomy. The increased c-fos mRNA expression and pain at postoperative 7 days was reduced by chemical sympathectomy. CONCLUSION: These results suggest that the transient gene expression of dynorphin and c-fos after tight ligation of L5 and L6 spinal nerves induces the development neuropathic pain, and late c-fos expression is related to neuropathic pain.
