Detection of the expression of p53 gene on retinal ganglion cells of rabbit eyes with intraocular hypertension
- VernacularTitle:实验性青光眼视网膜神经节细胞p53基因表达的检测
- Author:
Rongjiang, LUO
;
Jian, GE
;
Yehong, ZHUO
- Publication Type:Journal Article
- Keywords:
glaucoma;
retinal;
ganglion;
cells;
p53;
gene;
apoptosis
- From:
Chinese Journal of Experimental Ophthalmology
2001;19(3):208-210
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo detect the expression on p53 gene in retinal ganglion cells(RGCs) of rabbit eyes with different degrees of intraocular hypertension.MethodsIntraocular pressure(IOP)was elevated by intracameral injection of 0.10～0.15 ml of 2% methylcellulose.Animals were grouped up by different values of IOP.The rabbit eyes were enucleated,fixed,paraffin-embedded and cut into serial 6- μm thin sections.For histological evaluation,the slides were stained with DAB chromogen(conducting an immuohistochemical experiment),and were examined by light microscopic and automatic image analysis.ResultsThe number of RGCs with a positive reaction for p53 monoclonal antibody was statistically higher in the experimental eyes than in their control fellow eyes(P<0.01),and apoptosis was mainly found in the early stage of research.ConclusionOur research presents a new alternative view of apoptosis in experimental glaucoma(According to former reports,apoptosis was mainly found in the advanced stage of the patients with POAG)
