Effects of gamma-irradiation on intracellular proliferation of Toxoplasma gondii RH tachyzoites.
10.3347/kjp.1995.33.3.173
- Author:
Jina KOOK
;
Sung Hee OH
;
Chong Ku YUN
;
Jong Yil CHAI
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- MeSH:
Animal;
Cell Division/RADIATION EFFECTS;
Cells, Cultured;
Gamma Rays;
Human;
HL-60 Cells/PARASITOLOGY;
Macrophages/PARASITOLOGY;
Mice;
Mice, Inbred BALB C;
Mice, Inbred ICR;
Radiation Dosage;
Support, Non-U.S. Gov't;
Toxoplasma/*RADIATION EFFECTS/*CYTOLOGY
- From:The Korean Journal of Parasitology
1995;33(3):173-178
- CountryRepublic of Korea
- Language:English
-
Abstract:
A quantitative assay was performed on the effects of gamma-irradiation (30-300 Gy) on intracellular proliferation of Toxoplasma gondii RH tachyzoites in human leukemic HL-60 cells and murine peritoneal macrophages by means of 3H-uracil uptake assay. Infected non- irradiation group (NI) and uninfected group (incubating only host cells) were prepared. The 3H-uracil uptake by tachyzoites of NI group 12-24 hrs after infection was 2,190-4,787 counts per minute for macrophages and 2,967-8,254 for HL-60 cells, whereas the irradiated tachyzoites revealed only 381-703 (100 Gy) and 218-408 (300 Gy) for macrophages, and 1,911-2,618 (30 Gy), 1,253-1,384 (70 Gy), 1,013-1,090 (100 Gy), and 483-588 (300 Gy) for HL-60 cells. The proliferation inhibition rate was similar in macrophages and HL-60 cells, for example, 89-94% and 80-94% respectively by 300 Gy, 12-24 hrs after infection. It is concluded that RH tachyzoites of T. gondii are severely affected by gamma-irradiation in their capability of intracellular proliferation.
