A comparative study of 99mTc-YIGSR and 99mTc-MIBI uptake in tumor cells.
- Author:
Jia, HU
;
Xiaoli, LAN
;
Yongxue, ZHANG
;
Zairong, GAO
;
Jun, WU
- Publication Type:Journal Article
- MeSH:
Carcinoma, Ehrlich Tumor/*radionuclide imaging;
Laminin;
Oligopeptides;
Radiopharmaceuticals/diagnostic use;
Radiopharmaceuticals/*pharmacokinetics;
Technetium Tc 99m Mertiatide/diagnostic use;
Technetium Tc 99m Mertiatide/*pharmacokinetics;
Technetium Tc 99m Sestamibi/diagnostic use;
Technetium Tc 99m Sestamibi/*pharmacokinetics;
Tissue Distribution
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2005;25(6):712-4
- CountryChina
- Language:English
-
Abstract:
To investigate a new kind of tumor tracer 99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin -chain, which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG3. (1) Preparation of the 99mTc-YIGSR probe: with S-Acetly-NH3-MAG3 as the chelator and with proper reductants YIGSR was labeled with 99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EAC's uptake of 99mTc-YIGSR and 99mTc-MIBI was observed at 37 degrees C and 22 degrees C, respectively. (1) The labeling efficiencies of 99mTc-YIGSR and 99mTc-MIBI were (62 +/- 3)% and (96 +/- 2)%, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 degrees C, the EAC'S uptake of 99mTc-YIGSR and 99mTc-MIBI reached the peak of (43.16 +/- 2.4) % and (24.4 +/- 1.8) % at 60 min, respectively; and at 22 degrees C, the highest uptake was (26.5 +/- 2.1) % and (9.47 +/- 1.9) % at 60 min, respectively. The in vitro study suggests that 99mTc-YIGSR is superior to 99mTc-MIBI in cell uptake and has potential value in tumor imaging.
