Dark variants of luminous bacteria whole cell bioluminescent optical fiber sensor to genotoxicants.
- Author:
Yaliang, SUN
;
Tiebo, ZHOU
;
Jianli, GUO
;
Yiyong, LI
- Publication Type:Journal Article
- MeSH:
Biosensing Techniques;
Chemiluminescent Measurements;
Fiber Optics;
Luminescent Proteins/*genetics;
Mitomycin/*pharmacology;
Mitomycin/toxicity;
Photobacterium/*genetics;
Transcription, Genetic/drug effects;
Variation (Genetics)
- From:
Journal of Huazhong University of Science and Technology (Medical Sciences)
2004;24(5):507-9
- CountryChina
- Language:English
-
Abstract:
A stable dark variant separated from photobacterium phosphoreum (A2) was fixed in agar-gel membrane and immobilized onto an exposed end of a fiber-optic linked with bioluminometer. The variant could emit a luminescent signal in the presence of genotoxic agents, such as Mitomycin C (MC). The performance of this whole-cell optical fiber sensor system was examined as a function of several parameters, including gel probe thickness, bacterial cell density, and diameter of the fiber-optic core and working temperature. An optimal response to a model genotoxicant, Mitomycin C, was achieved with agar-bacterial gel membrane: the thickness of gel membrane was about 5 mm; the cell density of bacteria in gel membrane was about 2.0 x 10(7)/ml; the diameter of fiber-optic core was 5.0 mm; the working temperature was 25 degrees C. Under these optimized conditions, the response time was less than 10 h to Mitomycin C, with a lower detection threshold of 0.1 mg/L.
