Isolation and characterization of a new Methanosarcina mazei strain GFJ07 from a mountain forest pond
- Author:
Ya Li
;
ShengKui Lan
;
YuanPing Rong
;
QianWei Cheng
;
CuiJi Huang
;
ShiHua Wu1
;
Yi Yi
- Publication Type:Journal Article
- Keywords:
methanogen, archaea, Methanosarcina, Methanosarcina mazei, sausage-like
- From:Malaysian Journal of Microbiology
2012;8(1):6-10
- CountryMalaysia
- Language:English
-
Abstract:
Aims: A new methanogenic strain, named GFJ07, was isolated from a pond of mountain forests in Guangxi, China. Cells grown in liquid culture tended to form aggregates with pseudosarcina-like or irregular shape.
Methodology and Results: The optimum temperature, pH and NaCl concentration were 35 ℃, 7.0 and 0.5%, respectively. The isolate used methanol, trimethylamine, acetate and H2-CO2 as substrates. Analysis of the 16S rDNA sequences revealed strain GFJ07 showed the highest sequence similarity of 99.9% to Methanosarcina mazei.
Conclusion, significance and impact of study: The cells were Gram positive and nonmotile. Most of single cell grew as a sausage-like clinder about 0.5 μm in diameter and 1.0 μm in length.
- Full text:P020160923553688325123.pdf
