Comparison of the effect of arsenic sulfide on apoptosis and hTERT-mRNA expression in two leukemia cell lines
10.3321/j.issn:1007-8738.2009.10.011
- VernacularTitle:对比硫化砷对两种白血病细胞株凋亡和hTERT-mRNA表达的影响
- Author:
Jing LI
;
Yunxin CAO
;
Jinxia HAO
;
Shanxi LIU
- Publication Type:Journal Article
- Keywords:
arsenic sulfide;
telomerase;
cell cycle;
apoptosis;
K562 cell line;
NB4 cell line
- From:
Journal of Cellular and Molecular Immunology
2009;25(10):900-902,906
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To explore the different effect and mechanism of arsenic sulfide on telemorase activity and hTERT-mRNA expression in CML cell lines-KS62 and APL cell lines-NB4. METHODS: Telomerase activity was determined by polymerase chain reaction enzyme-linked immunoassay (PCR-ELISA). The expression of hTERT-mRNA was analyzed by semi-quantitative RT-PCR. Flow cytometry was used to analyze the cell cycle and apoptosis. RESULTS: 0.15-0.6 mg/L arsenic sulfide (72 h)can induce apoptosis and inhibit telomerase activity and hTERT-mRNA expression in NB4 cell. The concentration of arsenic sulfide with the same effect on K562 cell was 0.3-3 mg/U 0.3 mg/L arsenic sulfide (72 h) can cause the proportion of the NB4 cell in G2/M phase increased, but for K562 cell, The concentration of arsenic sulfide was 1.5 mg/L. CONCLUSION: Telomerase system may be one.of the pathway for arsenic sulfide inducing apoptosis of NB4 and K562 cell; G2/M phrase arrest may have correlation with decrease of telomerase activity; The sensitivity of NB4 and K562 call for arsenic sulfide is different, the mechanism of it need to study more.
