Effects of rosiglitazone on pancreatic β cells in a model of severe acute pancreatitis
10.3760/cma.j.issn.1674-6090.2013.03.003
- VernacularTitle:罗格列酮对重症急性胰腺炎胰岛β细胞作用的研究
- Author:
Chen CHEN
;
Liming LIU
;
Ying CHEN
;
Weixing WANG
;
Jia YU
;
Wenhong DENG
;
Bo YANG
- Publication Type:Journal Article
- Keywords:
Severe acute pancreatitis;
β cells;
Rosiglitazone;
Endocrine function
- From:
Journal of Endocrine Surgery
2013;7(3):184-186,190
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of rosiglitazone on pancreatic β cells in a rat model of severe acute pancreatitis(SAP).Methods 36 male Wistar rats were randomly divided into sham operation group(SO group),SAP group,and rosiglitazone pretreatment group(ROSI group).SAP model was induced by retrograde infusion of 5％ sodium taurocholate into the biliopancreatic duct.SO and SAP group were injected 10％ dimethyl sulfoxide partes aequales(DMSO) (0.2 ml/100 g)by femoral vein 30 mins piror to the surgery.ROSI group were injected rosiglitazone (6 mg/kg)instead of 10％ DMSO.Rats were killed 12 h after surgery and the level of serum amylase was detected.Pancreas tissues were taken for pathological examination under light microscope and insulin in situ staining was done.The ultrastructure of β cells was observed with transmission electron microscope.Results Amylase level and pathologic score were increased significantly in SAP group than in SO group (P ＜ 0.05).Compared with SAP group,amylase level and pathologic score reduced significantly for rats in ROSI group (P ＜ 0.05).Compared with SAP group,the morphological structure of β cells in ROSI group was more completed and insulin in situ staining was darker observed by electronic microscopy.Conclusion ROSI exerts protective effect on morphology and endocrine function of pancreatic β cells in rats with severe acute pancreatitis.
