Effects of midkine siRNA on adhesion and invasion of human breast cancer cells
10.3760/cma.j.issn.1674-6090.2011.03.002
- VernacularTitle:RNA干扰下调midkine基因表达对人乳腺癌细胞粘附和侵袭力的影响
- Author:
Li YU
;
Yu FAN
;
Zhiyuan QIU
;
Yongjing ZHOU
;
Dandan GONG
;
Xiudi XIAO
;
Zhengyan WU
- Publication Type:Journal Article
- Keywords:
Breast cancer;
Midkine;
RNA interference;
Adhesion;
Invasion
- From:
Journal of Endocrine Surgery
2011;05(3):148-151
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effects of midkine(MK)gene small interfering RNA(siRNA)on adhesion and invasion of human breast cancer cells.Methods Real time PCR was used to evaluate MK mRNA expression in 7 human breast cancer cell lines Bcap-37,LCCI,MCF-7,MDA-MB-231,MDA-MB-435,MDA-MB-468,and ZR75-1.The cell line in which MK expression was the highest was transfected with different doses of MK siRNA.The expression of MK mRNA and protein was determined by real-time quantitative PCR and immunoflurescence staining.The cell adhesion was evaluated by MTT assay and invasion was examined by Boyden chamber method.Results Cell line MCF-7 expressed the highestlevel of MK mRNA in the 7 tested breast cancer cell lines.After being transfected with MK siRNA,MK mRNA and protein level of MCF-7 decreased in timeand dose-dependent manners.The adhesive and invasive ability of MCF-7 cell transfected with MK siRNA decreased in a dose dependent manner(P<0.01,P<0.01).Conclusions MK gene might play an important role in adhesion and invasion of human breast cancer cells.siRNA transfection could effectively inhibit adhesion,migration,and invasion of human breast cancer cell.
