Determination of Rosolic Acid in Radix Rubi by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法测定托盘根药材中蔷薇酸的含量
- Author:
Dongyan CHENG
;
Lishu WANG
;
Xin CHEN
;
Jun GAO
;
Donghong CHENG
;
Chaonan WANG
- Keywords:
Radix Rubi;
Rosolic acid;
HPLC-ELSD;
Content determination
- From:
China Pharmacist
2017;20(8):1469-1470
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To establish an HPLC-ELSD method for the content determination of rosolic acid in Radix Rubi.Methods: The ELSD-HPLC content determination of rosolic acid was set up using a Vision HT C 18 HL column(250 mm× 4.6 mm ,5 μm), the mobile phase was methanol-0.1% trifluoroacetic acid with a flow rate of 0.8 ml·min-1 , the column temperature was 35℃, the temperature of drift tube heater was 90 ℃ and the gas was with the flow rate of 2.3 L ·min-1 .Results: The linear range of rosolic acid was 0.155-4.346 μg (r=0.999 9).The average recovery was 100.4% with RSD of 1.88% (n =6).Conclusion: The method is simple and accurate.It can be used for the quality control of Radix Rubi.
