Determination of dexamethasone in human plasma by solid phase extraction with ultra performance liquid chromatography-tandem mass spectrometer
10.13618/j.issn.1001-5728.2017.03.017
- VernacularTitle:固相萃取-液相色谱-质谱法检验人全血中的地塞米松
- Author:
Yujing LUAN
;
Ruihua WANG
;
Ying DONG
;
Leiping ZHANG
;
Zhongshan YU
- Keywords:
forensic toxicological analysis;
solid phase extraction;
UPLC-MS/MS;
dexamethasone
- From:
Chinese Journal of Forensic Medicine
2017;32(3):297-299
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop the analytical method to determine the content of dexamethasone in human plasma by solid phase extraction with ultra performance liquid chromatography-tandem mass spectrometer. Methods The human plasma was extracted with a solid phase extraction(SPE) and determined by UPLC-MS/MS. LC-MS/MS was performed in ESI source with MRM mode for quantification. Results The lowest detectable limit was 0.05ng/mL, the linear range was 1~100ng/mL. The absolute recovery was more than 78.1%. The intra- and inter day precision was within 15% at three concentrations. Conclusion Since the procedure proved to be simple, quick and effective, it could be used for the determination of dexamethasone in human plasma.
