Serum microRNA test in one Chinese early-onset familial Alzheimer's disease and preliminary analysis
10.3760/cma.j.issn.1674-6554.2017.06.015
- VernacularTitle:早发家族性阿尔茨海默病家系患者血清miRNA检测及初步分析
- Author:
Quanquan WANG
;
Zhanyun LYU
;
Dafang SUN
;
Xiaofu CAO
;
Yuzhong WANG
;
Yan YANG
;
Qingxia KONG
;
Yanlei HAO
- Keywords:
microRNA;
Early-onset familial Alzheimer's disease;
Microarray;
KEGG pathway analysis
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2017;26(6):554-559
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the expression profile of serum microRNAs(miRNAs) in early-onset familial Alzheimer's disease (EO-FAD) patients. methods miRNA microarrays were performed to detect the expression profile of serum miRNAs in 2 cases of EO-FAD patients,2 cases of EO-FAD carriers and 2 cases of normal controls.Preliminary bioinformatic analysis was conducted. Result sIt was found that 21 miRNAs were up-regulated and 22 miRNAs were down-regulated in serum of EO-FAD patients,the differences were statistically significant(P<0.05).miR-5704(P=0.0002),miR-4639-3p(P=0.0195),miR-107(P=0.0204) were markedly up-regulated,miR-5572(P=0.0008),miR-204-3p(P=0.0014),miR-542-5p(P=0.0106) and miR-155-5p(P=0.0240) were markedly down-regulated.Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis suggested that the dysregulated miRNAs may be involved in the mechanism of EO-FAD by affecting neurotrophin signaling pathway.Conclusion miR-5704,miR-4639-3p,miR-107,miR-5572,miR-204-3p,miR-542-5p and miR-155-5p may be used as potential biomarkers of EO-FAD,and involved in the mechanism of EO-FAD by affecting neurotrophin signaling pathway.
