Prevalence of Haplorchis taichui in Field-Collected Snails: A Molecular Approach.
10.3347/kjp.2010.48.4.343
- Author:
Thapana CHONTANANARTH
1
;
Chalobol WONGSAWAD
Author Information
1. Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50202, Thailand. cwongsawd@yahoo.com
- Publication Type:Brief Communication
- Keywords:
Haplorchis taichui;
pleurolophocercous cercaria;
thiarid snail;
PCR;
Thailand
- MeSH:
Animals;
DNA Primers/genetics;
DNA, Helminth/genetics;
Heterophyidae/*isolation & purification;
Microscopy/methods;
Parasitology/methods;
Polymerase Chain Reaction/methods;
Prevalence;
Snails/*parasitology;
Thailand
- From:The Korean Journal of Parasitology
2010;48(4):343-346
- CountryRepublic of Korea
- Language:English
-
Abstract:
The prevalence of the cercarial stage of an intestinal trematode, Haplorchis taichui, in thiarid snails (Gastropoda: Thiaridae) was investigated using light microscope and species-specific PCR procedures. A total of 988 snails were collected from Mae Taeng district, Chiang Mai province, northern Thailand, which comprised of 3 species; Melanoides tuberculata, Tarebia granifera, and Thiara scabra. The overall prevalence of pleurolophocercous cercariae was 21.7% as determined by the morphology. For genetic detection of H. taichui infection in snails, 2 primers Hapt_F (5'-GGCCAACGCAATCGTCATCC-3') and Hapt_R (5'-GCGTCGGGTTTCAGACATGG-3'), were used. The genomic DNA of H. taichui, which was used as a positive control, gave an amplification of the 256 bp fragment. The overall prevalence of H. taichui from specific PCR was 9.7%. The proportion of H. taichui among the pleurolophocercous cercariae in this study was 44.9%.
