Uncertainty Evaluation for the Determination of Salicylic Acid in Human Plasma by UPLC
10.3870/j.issn.1004-0781.2017.07.004
- VernacularTitle:超高效液相色谱法测定人血浆水杨酸浓度不确定度评定
- Author:
Kun ZHAO
;
Shenghui MEI
;
Xingang LI
;
Zhigang ZHAO
- Keywords:
Salicylic acid;
Uncertainty evaluation;
Chromatograph,ultra performance liquid;
Plasma,human
- From:
Herald of Medicine
2017;36(7):737-740
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the uncertainty for the determination of salicylic acid in human plasma by ultra performance liquid chromatography(UPLC).Methods All sources of uncertainty in the whole process of salicylic acid determination were analyzed,then the combined and expanded uncertainty were evaluated.Results The expanded uncertainty at concentrations of the lowest limit of quantitation(0.23 μg·mL-1) and a high level(39.43 μg·mL-1) of salicylic acid(P=95%,k=2) was 0.014 and 7.34 μg·mL-1,respectively.Conclusion The uncertainty of salicylic acid determination in human plasma by UPLC was mainly caused by recovery,repeatability and sample preparation at the lowest limit of quantitation and high qulity control concentration.
