MiR-124 involves in methamphetamineaddiction in PC12 cells by inhibiting GluR2
10.3969/j.issn.1001-1978.2017.07.019
- VernacularTitle:miR-124抑制GluR2参与甲基苯丙胺PC12细胞成瘾
- Author:
Mingjin JIANG
;
Duanwen CAO
;
Jian ZHOU
;
Yanni LYU
;
Yaling QIU
;
Jinhua WEN
;
Xiaohua WEI
- Keywords:
miR-124;
GluR2;
overexpression;
methamphetamine;
PC12 cells;
addiction;
AMPA
- From:
Chinese Pharmacological Bulletin
2017;33(7):982-986
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the change of miR-124 expression in methamphetamine-induced addiction in PC12 cells and the possible regulatory mechanism that it involves.Methods PC12 cells were randomly divided into 6 groups as follows: control group, methamphetamine group, agomir Negative Control group, miR-124 agomir group, agomir Negative Control+methamphetamine group and miR-124 agomir+methamphetamine group.After the treatment, the total RNA and protein were extracted in PC12 cells.The expression of miR-124 was measured by Real-time PCR and the expression of GluR2 was determined by Western blot in PC12 cells.Results Compared with those in the control group, the expression of miR-124 was remarkably decreased and the expression of GluR2 was significantly increased in the methamphetamine group in PC12 cells.Compared with those in the agomir Negative Control+methamphetamine group, the expression of miR-124 was remarkably increased and the expression of GluR2 was significantly decreased in the miR-124 agomir+methamphetamine group in PC12 cells.Conclusion MiR-124 might involve in methamphetamine-induced addiction in PC12 cells by inhibiting GluR2.
