Contents Determination of Luteoloside,Quercetin and Hyperoside in the Extract of Lonicera japonica by HPCE
10.6039/j.issn.1001-0408.2017.18.27
- VernacularTitle:HPCE法测定金银花药材中木犀草苷、槲皮素和金丝桃苷的含量
- Author:
Xue ZENG
;
Yuanjuan YANG
;
Zhu CHEN
;
Qian TANG
;
Lei SHI
- Keywords:
HPCE;
Luteoloside;
Quercetin;
Hyperoside;
Lonicera japonica
- From:
China Pharmacy
2017;28(18):2543-2545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for contents determination of luteoloside,quercetin and hyperoside in Lonicera japonica. METHODS:HPCE was performed silica capillary column with detection wavelength of 360 nm and separation voltage of 20 kV,electrokinetic sampling,sampling voltage of 15 kV,sampling time of 5 s,operation temperature of 25 ℃.The buffer was consisted of 60 mmol/L sodium tetraborate-50 mmol/L natrium carbonicum-50 mmol/L hydroxypropyl-β-cyclodextrin(pH 9.2). RE-SULTS:The linear ranges of luteoloside,quercetin and hyperoside were 0.06-0.56mg/mL (r=0.9881),0.08-0.56 mg/mL (r=0.9892),0.06-0.49 mg/mL(r=0.9796),respectively. RSDs of precision,stability and reproducibility tests were all lower than 2.0%. The recoveries were 96.12%-99.77%(RSD=1.29%,n=6),95.90%-98.35%(RSD=0.89%,n=6),94.07%-97.45%(RSD=1.33%,n=6),respectively. CONCLUSIONS:The method is simple,accurate,stable and reproducible,and can be used for simultaneous determination of luteoloside,quercetin and hyperoside in L. japonica.
