Simultaneous Determination of Six Saponins Constituents in Xinling Pills by HPLC-ELSD
- VernacularTitle:HPLC-ELSD法同时测定心灵丸中六种皂苷类成分的含量
- Author:
Jinxiang PENG
;
Feng WU
;
Huabin HUANG
;
Yuancui XU
;
Jin YANG
- Keywords:
Xinling pills;
HPLC-ELSD;
Notoginsenoside R1;
Ginsenoside Rg;
Ginsenoside Re;
Content determination
- From:
China Pharmacist
2017;20(6):1133-1135
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To develop an HPLC-ELSD method for the simultaneous determination of six saponins constituents including notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd in Xinling pills.Methods: HPLC-ELSD was used, and the chromatographic separation was performed on an Agilent Eclipse XBD-C18 column (150 mm×4.6 mm,5 μm) with acetonitrile-water as the mobile phase with gradient elution at the flow rate of 1.0 ml·min-1, the column temperature was maintained at 20℃, the drift tube temperature was 60℃, and the gas pressure was 4.00 bar.Results: Notoginsenoside R1, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rc and ginsenoside Rd was linear within the range of 0.30-6.00μg(r=0.999 5), 1.14-22.80μg (r=0.999 6), 0.17-3.40 μg (r=0.999 7), 0.81-16.20 μg (r=0.999 7), 0.08-1.60 μg (r=0.999 8) and 0.07-1.40 μg (r=0.999 8), respectively.The average recovery was 98.23%, 97.98%, 99.14%, 99.15%, 98.72% and 98.37%, and the RSDs were 1.56%, 1.31%, 1.16%, 1.07%, 0.73% and 0.92%(n=6), respectively.Conclusion: The method is convenient, accurate and reproducible in the quality control of saponins components in Xinling pills
