Cordycepin negatively modulates LPS-induced cytokine production by induction of Heme Oxygenase-1
10.3969/j.issn.1006-5725.2017.08.015
- VernacularTitle:虫草素诱导巨噬细胞表达血红素氧合酶-1负向调控细胞因子分泌
- Author:
Ni LI
;
Wei LIU
;
Lanhua ZHAO
;
Fan YANG
;
Ranhui LI
- Keywords:
Cordycepin;
Heme Oxygenase-1;
Murine macrophages
- From:
The Journal of Practical Medicine
2017;33(8):1250-1254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mocelualr mechanism of Cordycepin negative modulates LPSinduced cytokine production in murine macrophages.Methods The RAW264.7 murine macrophages were cultured in vitro and were pre-treated by different concentration of Cordycepin,and then stimulated by LPS for 8 h.Production of TNF-o,IL-6 and IL-12,and the content of p65 in the nuclear were detected by ELISA.Expression of heme oxygenase-1 (HO-1) and phosphorylation of IκB and p38 were measured by Western blot.Nuclear translocation of Nrf2 was detected by Immunofluorescence.Results 1 ～ 30 μg/mL of Cordycepin treatment significantly abrogated LPS-induced TNF-α,IL-6 and IL-12 production,p65 nuclear translocation and IκB phosphorylation.In addition,different concentration of Cordycepin could also induce RAW264.7 cells expression of HO-1,phosphorylation of p38 and nuclear translocation of Nrf2.Application of p38 inhibitor and small interfering RNA-mediated knock-down of Nrf-2 significantly inhibited surfactin-induced HO-1 expression.Treatment with a selective inhibitor of HO-1 reversed the Cordycepin mediated inhibition of pro-inflammatory cytokines.Conclusions Cordycepin induces antiinflammatory effects by inhibition of NF-κB and activation of Nrf-2 and p38 mediated HO-1 induction.
