Effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans
10.3969/j.issn.1002-2694.2017.06.003
- VernacularTitle:白念珠菌CDR1/CDR2和MDR1基因高表达对氧化应激的影响
- Author:
Ling JIANG
;
Yajing ZHAO
;
Shuixiu LI
;
Yanjun SONG
;
Hui GUO
;
Kunju ZHU
;
Hong ZHANG
- Keywords:
Candida albicans;
CDR1/CDR2 gene;
MDR1 gene;
overexpression;
oxidative stress
- From:
Chinese Journal of Zoonoses
2017;33(6):486-490
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effect of CDR1/CDR2 or MDR1 genes overexpression on oxidative stress in Candida albicans,we evaluated the effect of H2O2 on cell viability in C.albicans overexpressing genes CDR1 /CDR2 or MDR1 and their parent strains.After establishing an oxidative stress model with H2O2,we detected reactive oxygen species (ROS),mitochondrial membrane potential (Δψm) and the expression of oxidative stress response-related genes (CAP1 and GRP2) and ROS clearance related-genes (SOD2 and SOD5).The results showed that C.albicans growth were inhibited by 100％ after the treatment of 5 mmol/L H2O2.HeO2 caused more ROS accumulation and Δψm reduction in parent strains than in CDR1/CDR2 or MDR1 genes overexpressed strains (P＜0.05).Compared to parent strains,the up-regulated expression of CAP1 and GRP2 were relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains,moreover,the down-regulated expression of SOD2 and SOD5 were also relatively less in CDR1/CDR2 or MDR1 genes overexpressed strains (P＜0.05).In conclusion,the overexpression of CDR1/CDR2 and MDR1 genes could reduce the oxidative stress response and enhance the adaptability of C.albicans to oxidative stress.
